software for confocal microscope lsm 780 Search Results


zeiss invert 780  (Oxford Instruments)
99
Oxford Instruments zeiss invert 780
Zeiss Invert 780, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/software+for+confocal+microscope+lsm+780/Imaris/pmc10115647-257-19-25
Average 99 stars, based on 1 article reviews
zeiss invert 780 - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier
zeiss 780 confocal microscope  (Oxford Instruments)
99
Oxford Instruments zeiss 780 confocal microscope
Zeiss 780 Confocal Microscope, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/software+for+confocal+microscope+lsm+780/Dragonfly/pmc06562069-156-6-10
Average 99 stars, based on 1 article reviews
zeiss 780 confocal microscope - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier
fulvestrant  (Selleck Chemicals)
96
Selleck Chemicals fulvestrant
Estrogen-independent activation of AGR2 by IGF-1 requires estrogen receptor and activation of ERK and AKT pathways. a , b and d , e MCF7 cells were incubated for 24 h in different treatments with IGF-1 and MEK, ER and AKT inhibitors. The concentration of IGF-1 is 10 nM in each group. MEK inhibitor U0126 (10 μM), ER inhibitor <t>ICI</t> <t>182,</t> <t>780</t> (10 nM) and AKT inhibitor MK2206 (500 nM) and E2 (5 nM) were used. Level of AGR2 protein was detected by western blot with specific antibodies. β-Actin was detected as a loading control. c , f Luciferase construct with 1.9 kb of AGR2 promoter sequence and a pRL-TK control plasmid was co-transfected into MCF7 cells and treated with different inhibitors with or without the presence of 10 nM IGF-1 and 5 μM E2. The luciferase activity was detected with microplate reader and normalized by Renilla activity. g AGR2 expression induced by IGF-1 without E2 in the presence of four chemical inhibitors was detected by immunofluorescence in MCF7 cell by confocal microscopy. The inhibitor concentration was 10 μM U0126, 10 nM ICI 182,780, 500 nM MK2206 and 5 μM OSI-906. Cells were starved for 24 h before IGF-1 treatment. Nuclei were stained with DAPI ( blue ) as an internal reference, and AGR2 was stained with specific primary antibody ( red ). The IOD value of red fluorescence is quantified by Image-Pro, normalized to blue fluorescence and set in the right panel in the form of a histogram. The original magnification is ×200. Each experiment was repeated at least three times. * P < 0.05; ** P < 0.01
Fulvestrant, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/software+for+confocal+microscope+lsm+780/Fulvestrant/pmc04451465-54-8-33
Average 96 stars, based on 1 article reviews
fulvestrant - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
axio observer z1 microscope  (Carl Zeiss)
99
Carl Zeiss axio observer z1 microscope
Estrogen-independent activation of AGR2 by IGF-1 requires estrogen receptor and activation of ERK and AKT pathways. a , b and d , e MCF7 cells were incubated for 24 h in different treatments with IGF-1 and MEK, ER and AKT inhibitors. The concentration of IGF-1 is 10 nM in each group. MEK inhibitor U0126 (10 μM), ER inhibitor <t>ICI</t> <t>182,</t> <t>780</t> (10 nM) and AKT inhibitor MK2206 (500 nM) and E2 (5 nM) were used. Level of AGR2 protein was detected by western blot with specific antibodies. β-Actin was detected as a loading control. c , f Luciferase construct with 1.9 kb of AGR2 promoter sequence and a pRL-TK control plasmid was co-transfected into MCF7 cells and treated with different inhibitors with or without the presence of 10 nM IGF-1 and 5 μM E2. The luciferase activity was detected with microplate reader and normalized by Renilla activity. g AGR2 expression induced by IGF-1 without E2 in the presence of four chemical inhibitors was detected by immunofluorescence in MCF7 cell by confocal microscopy. The inhibitor concentration was 10 μM U0126, 10 nM ICI 182,780, 500 nM MK2206 and 5 μM OSI-906. Cells were starved for 24 h before IGF-1 treatment. Nuclei were stained with DAPI ( blue ) as an internal reference, and AGR2 was stained with specific primary antibody ( red ). The IOD value of red fluorescence is quantified by Image-Pro, normalized to blue fluorescence and set in the right panel in the form of a histogram. The original magnification is ×200. Each experiment was repeated at least three times. * P < 0.05; ** P < 0.01
Axio Observer Z1 Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/software+for+confocal+microscope+lsm+780/Inverted+microscope+Axio+Observer+3/bio_rxiv__2022__01__29__478341-334-10-9
Average 99 stars, based on 1 article reviews
axio observer z1 microscope - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier
780 confocal laser scanning inverted microscope  (Carl Zeiss)
99
Carl Zeiss 780 confocal laser scanning inverted microscope
Estrogen-independent activation of AGR2 by IGF-1 requires estrogen receptor and activation of ERK and AKT pathways. a , b and d , e MCF7 cells were incubated for 24 h in different treatments with IGF-1 and MEK, ER and AKT inhibitors. The concentration of IGF-1 is 10 nM in each group. MEK inhibitor U0126 (10 μM), ER inhibitor <t>ICI</t> <t>182,</t> <t>780</t> (10 nM) and AKT inhibitor MK2206 (500 nM) and E2 (5 nM) were used. Level of AGR2 protein was detected by western blot with specific antibodies. β-Actin was detected as a loading control. c , f Luciferase construct with 1.9 kb of AGR2 promoter sequence and a pRL-TK control plasmid was co-transfected into MCF7 cells and treated with different inhibitors with or without the presence of 10 nM IGF-1 and 5 μM E2. The luciferase activity was detected with microplate reader and normalized by Renilla activity. g AGR2 expression induced by IGF-1 without E2 in the presence of four chemical inhibitors was detected by immunofluorescence in MCF7 cell by confocal microscopy. The inhibitor concentration was 10 μM U0126, 10 nM ICI 182,780, 500 nM MK2206 and 5 μM OSI-906. Cells were starved for 24 h before IGF-1 treatment. Nuclei were stained with DAPI ( blue ) as an internal reference, and AGR2 was stained with specific primary antibody ( red ). The IOD value of red fluorescence is quantified by Image-Pro, normalized to blue fluorescence and set in the right panel in the form of a histogram. The original magnification is ×200. Each experiment was repeated at least three times. * P < 0.05; ** P < 0.01
780 Confocal Laser Scanning Inverted Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/software+for+confocal+microscope+lsm+780/Primovert+Compact+Inverted+Microscope/pm42854796-272-19-18
Average 99 stars, based on 1 article reviews
780 confocal laser scanning inverted microscope - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier
ti 2 yokogawa w1 spinning disk confocal microscope  (Yokogawa Electric)
99
Yokogawa Electric ti 2 yokogawa w1 spinning disk confocal microscope
Estrogen-independent activation of AGR2 by IGF-1 requires estrogen receptor and activation of ERK and AKT pathways. a , b and d , e MCF7 cells were incubated for 24 h in different treatments with IGF-1 and MEK, ER and AKT inhibitors. The concentration of IGF-1 is 10 nM in each group. MEK inhibitor U0126 (10 μM), ER inhibitor <t>ICI</t> <t>182,</t> <t>780</t> (10 nM) and AKT inhibitor MK2206 (500 nM) and E2 (5 nM) were used. Level of AGR2 protein was detected by western blot with specific antibodies. β-Actin was detected as a loading control. c , f Luciferase construct with 1.9 kb of AGR2 promoter sequence and a pRL-TK control plasmid was co-transfected into MCF7 cells and treated with different inhibitors with or without the presence of 10 nM IGF-1 and 5 μM E2. The luciferase activity was detected with microplate reader and normalized by Renilla activity. g AGR2 expression induced by IGF-1 without E2 in the presence of four chemical inhibitors was detected by immunofluorescence in MCF7 cell by confocal microscopy. The inhibitor concentration was 10 μM U0126, 10 nM ICI 182,780, 500 nM MK2206 and 5 μM OSI-906. Cells were starved for 24 h before IGF-1 treatment. Nuclei were stained with DAPI ( blue ) as an internal reference, and AGR2 was stained with specific primary antibody ( red ). The IOD value of red fluorescence is quantified by Image-Pro, normalized to blue fluorescence and set in the right panel in the form of a histogram. The original magnification is ×200. Each experiment was repeated at least three times. * P < 0.05; ** P < 0.01
Ti 2 Yokogawa W1 Spinning Disk Confocal Microscope, supplied by Yokogawa Electric, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/software+for+confocal+microscope+lsm+780/CSU-W1/pmc12506632-123-19-21
Average 99 stars, based on 1 article reviews
ti 2 yokogawa w1 spinning disk confocal microscope - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier
chameleon nir tunable laser  (Coherent Corp)
99
Coherent Corp chameleon nir tunable laser
Estrogen-independent activation of AGR2 by IGF-1 requires estrogen receptor and activation of ERK and AKT pathways. a , b and d , e MCF7 cells were incubated for 24 h in different treatments with IGF-1 and MEK, ER and AKT inhibitors. The concentration of IGF-1 is 10 nM in each group. MEK inhibitor U0126 (10 μM), ER inhibitor <t>ICI</t> <t>182,</t> <t>780</t> (10 nM) and AKT inhibitor MK2206 (500 nM) and E2 (5 nM) were used. Level of AGR2 protein was detected by western blot with specific antibodies. β-Actin was detected as a loading control. c , f Luciferase construct with 1.9 kb of AGR2 promoter sequence and a pRL-TK control plasmid was co-transfected into MCF7 cells and treated with different inhibitors with or without the presence of 10 nM IGF-1 and 5 μM E2. The luciferase activity was detected with microplate reader and normalized by Renilla activity. g AGR2 expression induced by IGF-1 without E2 in the presence of four chemical inhibitors was detected by immunofluorescence in MCF7 cell by confocal microscopy. The inhibitor concentration was 10 μM U0126, 10 nM ICI 182,780, 500 nM MK2206 and 5 μM OSI-906. Cells were starved for 24 h before IGF-1 treatment. Nuclei were stained with DAPI ( blue ) as an internal reference, and AGR2 was stained with specific primary antibody ( red ). The IOD value of red fluorescence is quantified by Image-Pro, normalized to blue fluorescence and set in the right panel in the form of a histogram. The original magnification is ×200. Each experiment was repeated at least three times. * P < 0.05; ** P < 0.01
Chameleon Nir Tunable Laser, supplied by Coherent Corp, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/software+for+confocal+microscope+lsm+780/Chameleon/pmc08967408-404-14-13
Average 99 stars, based on 1 article reviews
chameleon nir tunable laser - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier
laser scanning confocal microscopy  (Olympus)
99
Olympus laser scanning confocal microscopy
Estrogen-independent activation of AGR2 by IGF-1 requires estrogen receptor and activation of ERK and AKT pathways. a , b and d , e MCF7 cells were incubated for 24 h in different treatments with IGF-1 and MEK, ER and AKT inhibitors. The concentration of IGF-1 is 10 nM in each group. MEK inhibitor U0126 (10 μM), ER inhibitor <t>ICI</t> <t>182,</t> <t>780</t> (10 nM) and AKT inhibitor MK2206 (500 nM) and E2 (5 nM) were used. Level of AGR2 protein was detected by western blot with specific antibodies. β-Actin was detected as a loading control. c , f Luciferase construct with 1.9 kb of AGR2 promoter sequence and a pRL-TK control plasmid was co-transfected into MCF7 cells and treated with different inhibitors with or without the presence of 10 nM IGF-1 and 5 μM E2. The luciferase activity was detected with microplate reader and normalized by Renilla activity. g AGR2 expression induced by IGF-1 without E2 in the presence of four chemical inhibitors was detected by immunofluorescence in MCF7 cell by confocal microscopy. The inhibitor concentration was 10 μM U0126, 10 nM ICI 182,780, 500 nM MK2206 and 5 μM OSI-906. Cells were starved for 24 h before IGF-1 treatment. Nuclei were stained with DAPI ( blue ) as an internal reference, and AGR2 was stained with specific primary antibody ( red ). The IOD value of red fluorescence is quantified by Image-Pro, normalized to blue fluorescence and set in the right panel in the form of a histogram. The original magnification is ×200. Each experiment was repeated at least three times. * P < 0.05; ** P < 0.01
Laser Scanning Confocal Microscopy, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/software+for+confocal+microscope+lsm+780/FV3000+Confocal+Laser+Scanning+Microscope/pmc11663453-111-10-15
Average 99 stars, based on 1 article reviews
laser scanning confocal microscopy - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier
confocal microscope  (Nikon)
99
Nikon confocal microscope
Estrogen-independent activation of AGR2 by IGF-1 requires estrogen receptor and activation of ERK and AKT pathways. a , b and d , e MCF7 cells were incubated for 24 h in different treatments with IGF-1 and MEK, ER and AKT inhibitors. The concentration of IGF-1 is 10 nM in each group. MEK inhibitor U0126 (10 μM), ER inhibitor <t>ICI</t> <t>182,</t> <t>780</t> (10 nM) and AKT inhibitor MK2206 (500 nM) and E2 (5 nM) were used. Level of AGR2 protein was detected by western blot with specific antibodies. β-Actin was detected as a loading control. c , f Luciferase construct with 1.9 kb of AGR2 promoter sequence and a pRL-TK control plasmid was co-transfected into MCF7 cells and treated with different inhibitors with or without the presence of 10 nM IGF-1 and 5 μM E2. The luciferase activity was detected with microplate reader and normalized by Renilla activity. g AGR2 expression induced by IGF-1 without E2 in the presence of four chemical inhibitors was detected by immunofluorescence in MCF7 cell by confocal microscopy. The inhibitor concentration was 10 μM U0126, 10 nM ICI 182,780, 500 nM MK2206 and 5 μM OSI-906. Cells were starved for 24 h before IGF-1 treatment. Nuclei were stained with DAPI ( blue ) as an internal reference, and AGR2 was stained with specific primary antibody ( red ). The IOD value of red fluorescence is quantified by Image-Pro, normalized to blue fluorescence and set in the right panel in the form of a histogram. The original magnification is ×200. Each experiment was repeated at least three times. * P < 0.05; ** P < 0.01
Confocal Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/software+for+confocal+microscope+lsm+780/Objectives/10__1523_slash_jneurosci__0184___19__2019-113-8-10
Average 99 stars, based on 1 article reviews
confocal microscope - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier
mll  (Bethyl)
95
Bethyl mll
Immunofluorescence antibody patterns of selected DFS70/LEDGFp75 interacting partners in HEp-2 cells. ( A ) The IFA patterns of rabbit antibodies to the DFS70/LEDGFp75 interacting partners Menin, POGZ, JOP2, c-MYC, and IWS1 show nuclear dense fine speckles without strong and well-defined mitotic chromatin staining. HRP2 showed mitotic spindle staining without nuclear dense fine speckles. <t>MLL</t> <t>and</t> <t>H3K36me2</t> showed both nuclear dense fine speckles and intense mitotic chromatin staining.Yellow arrows point to mitotic cells. ( B ) Pre-adsorption of the rabbit antibodies to DFS70/LEDGFp75 interacting partners with the IBD autoepitope peptide did not abolish their immunoreactivity, ruling out the possibility that these antibodies may be cross-reacting with the DFS70/LEDGFp75 IBD region. Fluorescent images were obtained using a Keyence Biorevo microscope. Chromatin staining with DAPI is presented in black and white for better visualization.
Mll, supplied by Bethyl, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/software+for+confocal+microscope+lsm+780/MLL1+Antibody/pmc09858485-52-74-75
Average 95 stars, based on 1 article reviews
mll - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
confocal microscope  (Danaher Inc)
99
Danaher Inc confocal microscope
Immunofluorescence antibody patterns of selected DFS70/LEDGFp75 interacting partners in HEp-2 cells. ( A ) The IFA patterns of rabbit antibodies to the DFS70/LEDGFp75 interacting partners Menin, POGZ, JOP2, c-MYC, and IWS1 show nuclear dense fine speckles without strong and well-defined mitotic chromatin staining. HRP2 showed mitotic spindle staining without nuclear dense fine speckles. <t>MLL</t> <t>and</t> <t>H3K36me2</t> showed both nuclear dense fine speckles and intense mitotic chromatin staining.Yellow arrows point to mitotic cells. ( B ) Pre-adsorption of the rabbit antibodies to DFS70/LEDGFp75 interacting partners with the IBD autoepitope peptide did not abolish their immunoreactivity, ruling out the possibility that these antibodies may be cross-reacting with the DFS70/LEDGFp75 IBD region. Fluorescent images were obtained using a Keyence Biorevo microscope. Chromatin staining with DAPI is presented in black and white for better visualization.
Confocal Microscope, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/software+for+confocal+microscope+lsm+780/STELLARIS+5+Confocal+Microscope+Platforms/pmc12211844-46-60-64
Average 99 stars, based on 1 article reviews
confocal microscope - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier
confocal microscope  (Carl Zeiss)
94
Carl Zeiss confocal microscope
Immunofluorescence antibody patterns of selected DFS70/LEDGFp75 interacting partners in HEp-2 cells. ( A ) The IFA patterns of rabbit antibodies to the DFS70/LEDGFp75 interacting partners Menin, POGZ, JOP2, c-MYC, and IWS1 show nuclear dense fine speckles without strong and well-defined mitotic chromatin staining. HRP2 showed mitotic spindle staining without nuclear dense fine speckles. <t>MLL</t> <t>and</t> <t>H3K36me2</t> showed both nuclear dense fine speckles and intense mitotic chromatin staining.Yellow arrows point to mitotic cells. ( B ) Pre-adsorption of the rabbit antibodies to DFS70/LEDGFp75 interacting partners with the IBD autoepitope peptide did not abolish their immunoreactivity, ruling out the possibility that these antibodies may be cross-reacting with the DFS70/LEDGFp75 IBD region. Fluorescent images were obtained using a Keyence Biorevo microscope. Chromatin staining with DAPI is presented in black and white for better visualization.
Confocal Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/software+for+confocal+microscope+lsm+780/Objective+EC+Plan-Neofluar+10x%2F0%2E3+M27/bio_rxiv__64898__2026__03__17__712327-264-7-11
Average 94 stars, based on 1 article reviews
confocal microscope - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier

Image Search Results


Estrogen-independent activation of AGR2 by IGF-1 requires estrogen receptor and activation of ERK and AKT pathways. a , b and d , e MCF7 cells were incubated for 24 h in different treatments with IGF-1 and MEK, ER and AKT inhibitors. The concentration of IGF-1 is 10 nM in each group. MEK inhibitor U0126 (10 μM), ER inhibitor ICI 182, 780 (10 nM) and AKT inhibitor MK2206 (500 nM) and E2 (5 nM) were used. Level of AGR2 protein was detected by western blot with specific antibodies. β-Actin was detected as a loading control. c , f Luciferase construct with 1.9 kb of AGR2 promoter sequence and a pRL-TK control plasmid was co-transfected into MCF7 cells and treated with different inhibitors with or without the presence of 10 nM IGF-1 and 5 μM E2. The luciferase activity was detected with microplate reader and normalized by Renilla activity. g AGR2 expression induced by IGF-1 without E2 in the presence of four chemical inhibitors was detected by immunofluorescence in MCF7 cell by confocal microscopy. The inhibitor concentration was 10 μM U0126, 10 nM ICI 182,780, 500 nM MK2206 and 5 μM OSI-906. Cells were starved for 24 h before IGF-1 treatment. Nuclei were stained with DAPI ( blue ) as an internal reference, and AGR2 was stained with specific primary antibody ( red ). The IOD value of red fluorescence is quantified by Image-Pro, normalized to blue fluorescence and set in the right panel in the form of a histogram. The original magnification is ×200. Each experiment was repeated at least three times. * P < 0.05; ** P < 0.01

Journal: Medical Oncology (Northwood, London, England)

Article Title: Induction of anterior gradient 2 (AGR2) plays a key role in insulin-like growth factor-1 (IGF-1)-induced breast cancer cell proliferation and migration

doi: 10.1007/s12032-015-0577-z

Figure Lengend Snippet: Estrogen-independent activation of AGR2 by IGF-1 requires estrogen receptor and activation of ERK and AKT pathways. a , b and d , e MCF7 cells were incubated for 24 h in different treatments with IGF-1 and MEK, ER and AKT inhibitors. The concentration of IGF-1 is 10 nM in each group. MEK inhibitor U0126 (10 μM), ER inhibitor ICI 182, 780 (10 nM) and AKT inhibitor MK2206 (500 nM) and E2 (5 nM) were used. Level of AGR2 protein was detected by western blot with specific antibodies. β-Actin was detected as a loading control. c , f Luciferase construct with 1.9 kb of AGR2 promoter sequence and a pRL-TK control plasmid was co-transfected into MCF7 cells and treated with different inhibitors with or without the presence of 10 nM IGF-1 and 5 μM E2. The luciferase activity was detected with microplate reader and normalized by Renilla activity. g AGR2 expression induced by IGF-1 without E2 in the presence of four chemical inhibitors was detected by immunofluorescence in MCF7 cell by confocal microscopy. The inhibitor concentration was 10 μM U0126, 10 nM ICI 182,780, 500 nM MK2206 and 5 μM OSI-906. Cells were starved for 24 h before IGF-1 treatment. Nuclei were stained with DAPI ( blue ) as an internal reference, and AGR2 was stained with specific primary antibody ( red ). The IOD value of red fluorescence is quantified by Image-Pro, normalized to blue fluorescence and set in the right panel in the form of a histogram. The original magnification is ×200. Each experiment was repeated at least three times. * P < 0.05; ** P < 0.01

Article Snippet: In addition, chemical inhibitors included U0126-EtOH (MEK inhibitor), fulvestrant (ICI 182,780, estrogen receptor-α inhibitor) and MK2206 2HCl (AKT inhibitor), and SP00125 (JNK Inhibitor) and OSI-906 (IGF-1 receptor inhibitor), which were also purchased from Selleckchem, while monensin was from Beyotime.

Techniques: Activation Assay, Incubation, Concentration Assay, Western Blot, Control, Luciferase, Construct, Sequencing, Plasmid Preparation, Transfection, Activity Assay, Expressing, Immunofluorescence, Confocal Microscopy, Staining, Fluorescence

Immunofluorescence antibody patterns of selected DFS70/LEDGFp75 interacting partners in HEp-2 cells. ( A ) The IFA patterns of rabbit antibodies to the DFS70/LEDGFp75 interacting partners Menin, POGZ, JOP2, c-MYC, and IWS1 show nuclear dense fine speckles without strong and well-defined mitotic chromatin staining. HRP2 showed mitotic spindle staining without nuclear dense fine speckles. MLL and H3K36me2 showed both nuclear dense fine speckles and intense mitotic chromatin staining.Yellow arrows point to mitotic cells. ( B ) Pre-adsorption of the rabbit antibodies to DFS70/LEDGFp75 interacting partners with the IBD autoepitope peptide did not abolish their immunoreactivity, ruling out the possibility that these antibodies may be cross-reacting with the DFS70/LEDGFp75 IBD region. Fluorescent images were obtained using a Keyence Biorevo microscope. Chromatin staining with DAPI is presented in black and white for better visualization.

Journal: Diagnostics

Article Title: The Nuclear Dense Fine Speckled (DFS) Immunofluorescence Pattern: Not All Roads Lead to DFS70/LEDGFp75

doi: 10.3390/diagnostics13020222

Figure Lengend Snippet: Immunofluorescence antibody patterns of selected DFS70/LEDGFp75 interacting partners in HEp-2 cells. ( A ) The IFA patterns of rabbit antibodies to the DFS70/LEDGFp75 interacting partners Menin, POGZ, JOP2, c-MYC, and IWS1 show nuclear dense fine speckles without strong and well-defined mitotic chromatin staining. HRP2 showed mitotic spindle staining without nuclear dense fine speckles. MLL and H3K36me2 showed both nuclear dense fine speckles and intense mitotic chromatin staining.Yellow arrows point to mitotic cells. ( B ) Pre-adsorption of the rabbit antibodies to DFS70/LEDGFp75 interacting partners with the IBD autoepitope peptide did not abolish their immunoreactivity, ruling out the possibility that these antibodies may be cross-reacting with the DFS70/LEDGFp75 IBD region. Fluorescent images were obtained using a Keyence Biorevo microscope. Chromatin staining with DAPI is presented in black and white for better visualization.

Article Snippet: We also used in this study commercially available rabbit antibodies to DFS70/LEDGFp75 (Bethyl Laboratories/ Fortis Life Sciences, Montgomery, TX, USA, Cat# A300-848A), Menin (Bethyl Laboratories/Fortis Life Sciences, Cat# A300-105A), JPO2 (Bethyl Laboratories/Fortis Life Sciences, Cat# A300-846A), HRP2 (Bethyl Laboratories/Fortis Life Sciences, Cat# A304-314A), IWS1 (Cell Signaling Technology, Danvers, MA, USA, Cat#5681), c-MYC (Cell Signaling Technology, Cat# 18583), PogZ (Aviva Systems Biology, San Diego, CA, USA Cat# RP39173-P050), H3K36me2 (Cell Signaling Technology, Cat# 2901T), and MLL (Bethyl Laboratories/Fortis Life Sciences, Cat# A300-086A).

Techniques: Immunofluorescence, Staining, Adsorption, Microscopy

Co-localization of monospecific CLIA anti-DFS70-positive and anti-DFS70-negative autoantibodies with anti-MLL and anti-H3K36me2 antibodies. ( A ) CLIA anti-DFS70-positive anti-DFS70/LEDGFp75 serum PL61 (green) was co-incubated with rabbit antibodies to MLL or H3K36me2 (red) in the HEp-2 IFA test. Co-localization between DFS70/LEDGFp75 and its known interacting partners MLL (IBD region) and H3K36me2 (PWWP domain) is observed, as evidenced by the extensive yellow/orange fluorescence of the merged images. ( B ) CLIA anti-DFS70-negative serum PL24 was also co-incubated with rabbit antibodies to MLL or H3K36me2. Areas of strong co-localization between the pseudo-DFS pattern produced by serum PL24 and the patterns produced by the anti-MLL and H3K36me2 rabbit antibodies can be detected (yellow/orange fluorescence in merged images). However, the co-localization is not as extensive as that shown in panel A given the increased separation of red and green fluorescence in the merged images. Images were obtained by confocal microscopy. Chromatin staining with DAPI is presented in black and white for better visualization.

Journal: Diagnostics

Article Title: The Nuclear Dense Fine Speckled (DFS) Immunofluorescence Pattern: Not All Roads Lead to DFS70/LEDGFp75

doi: 10.3390/diagnostics13020222

Figure Lengend Snippet: Co-localization of monospecific CLIA anti-DFS70-positive and anti-DFS70-negative autoantibodies with anti-MLL and anti-H3K36me2 antibodies. ( A ) CLIA anti-DFS70-positive anti-DFS70/LEDGFp75 serum PL61 (green) was co-incubated with rabbit antibodies to MLL or H3K36me2 (red) in the HEp-2 IFA test. Co-localization between DFS70/LEDGFp75 and its known interacting partners MLL (IBD region) and H3K36me2 (PWWP domain) is observed, as evidenced by the extensive yellow/orange fluorescence of the merged images. ( B ) CLIA anti-DFS70-negative serum PL24 was also co-incubated with rabbit antibodies to MLL or H3K36me2. Areas of strong co-localization between the pseudo-DFS pattern produced by serum PL24 and the patterns produced by the anti-MLL and H3K36me2 rabbit antibodies can be detected (yellow/orange fluorescence in merged images). However, the co-localization is not as extensive as that shown in panel A given the increased separation of red and green fluorescence in the merged images. Images were obtained by confocal microscopy. Chromatin staining with DAPI is presented in black and white for better visualization.

Article Snippet: We also used in this study commercially available rabbit antibodies to DFS70/LEDGFp75 (Bethyl Laboratories/ Fortis Life Sciences, Montgomery, TX, USA, Cat# A300-848A), Menin (Bethyl Laboratories/Fortis Life Sciences, Cat# A300-105A), JPO2 (Bethyl Laboratories/Fortis Life Sciences, Cat# A300-846A), HRP2 (Bethyl Laboratories/Fortis Life Sciences, Cat# A304-314A), IWS1 (Cell Signaling Technology, Danvers, MA, USA, Cat#5681), c-MYC (Cell Signaling Technology, Cat# 18583), PogZ (Aviva Systems Biology, San Diego, CA, USA Cat# RP39173-P050), H3K36me2 (Cell Signaling Technology, Cat# 2901T), and MLL (Bethyl Laboratories/Fortis Life Sciences, Cat# A300-086A).

Techniques: Incubation, Fluorescence, Produced, Confocal Microscopy, Staining